Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore. Issue 45 (22nd May 2017)

• Record Type: Journal Article
• Authors: Collot, Mayeul; Ponsot, Flavien; Klymchenko, Andrey S.
• Is Part Of: Chemical communications. Volume 53:Issue 45(2017)
• Page Start: 6117
• Page End: 6120
• Publication Date: 2017-05-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc02418e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Ca-NIR is the first ratiometric fluorescent calcium probe emitting in the near infrared. Abstract : Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630–640 nm lasers and displays two distinct emission bands depending on the calcium concentration ( K d = ∼8 μM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.