Comparison of manual and automatic processing of biological samples for electron microscopy. Issue 6 (8th February 2017)

• Record Type: Journal Article
• Authors: Yang, Rui; Wang, Shuai; Wang, Jianli; Luo, Xi; Zhao, Wenchao; Zhang, Qing; Nan, Zhangjie; Yang, Liu; Wang, Shaohui
• Is Part Of: Microscopy research and technique. Volume 80:Issue 6(2017)
• Page Start: 570
• Page End: 577
• Publication Date: 2017-02-08
• Subjects: automation -- sample preparation -- SEM -- TEM; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22832
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: The ultrastructure of a sample can be observed by electron microscopy (EM), which has become an indispensable research tool in morphological studies. However, EM sample preparation techniques are complicated and time‐consuming, with a high labor cost. The current study was conducted to compare the conventional manual and automated methods for sample processing and post‐staining for electron microscopy. Automated sample processing reduces OsO4 contamination, improves the efficiency of sample preparation and is easy to use. Therefore, the results of their study provide a practical and feasible method for the preparation of biological samples for electron microscopy. Abstract : The ultrastructure of a sample can be observed by electron microscopy (EM), which has become an indispensable research tool in morphological studies. However, EM sample preparation techniques are complicated and time‐consuming, with a high labor cost. The current study was conducted to compare the conventional manual and automated methods for sample processing and post‐staining for electron microscopy. Automated sample processing reduces OsO4 contamination, improves the efficiency of sample preparation and is easy to use. Therefore, the results of our study provide a practical and feasible method for the preparation of biological samples for electron microscopy.