Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post‐harvest Citrus Fruit. Issue 11 (November 2013)

• Record Type: Journal Article
• Authors: Sun, Xiaohua; Zhu, Andan; Liu, Shuzhen; Sheng, Ling; Ma, Qiaoli; Zhang, Li; Nishawy, Elsayed Mohamed Elsayed; Zeng, Yunliu; Xu, Juan; Ma, Zhaocheng; Cheng, Yunjiang; Deng, Xiuxin
• Is Part Of: Journal of integrative plant biology. Volume 55:Issue 11(2013)
• Page Start: 1038
• Page End: 1053
• Publication Date: 2013-11
• Subjects: Citrus -- gene expression -- metabolite -- organic acids -- post‐harvest storage; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12083
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: Citric acid plays an important role in fresh fruit flavor and its adaptability to post‐harvest storage conditions. In order to explore organic acid regulatory mechanisms in post‐harvest citrus fruit, systematic biological analyses were conducted on stored Hirado Buntan Pummelo (HBP; Citrus grandis ) fruits. High‐performance capillary electrophoresis, subcellular organelle expression microarray, real‐time quantitative reverse transcription polymerase chain reaction, gas chromatography mass spectrometry (GC‐MS), and conventional physiological and biochemical analyses were undertaken. The results showed that the concentration of organic acids in HBP underwent a regular fluctuation. GC‐MS‐based metabolic profiling indicated that succinic acid, γ‐aminobutyric acid (GABA), and glutamine contents increased, but 2‐oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes. In addition, the concentration of organic acids was significantly correlated with senescence‐related physiological processes, such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities, which showed that organic acids could be regarded as important parameters for measuring citrus fruit post‐harvest senescence processes.