Characterization of high hydrostatic pressure-injured Bacillus subtilis cells*. Issue 6 (3rd June 2017)

• Record Type: Journal Article
• Authors: Inaoka, Takashi; Kimura, Keitarou; Morimatsu, Kazuya; Yamamoto, Kazutaka
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 6(2017)
• Page Start: 1235
• Page End: 1240
• Publication Date: 2017-06-03
• Subjects: high hydrostatic pressure -- injured cells -- Bacillus subtilis; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1292835
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract : High hydrostatic pressure (HHP) affects various cellular processes. Using a sporulation-deficient Bacillus subtilis strain, we characterized the properties of vegetative cells subjected to HHP. When stationary-phase cells were exposed to 250 MPa of HHP for 10 min at 25 °C, approximately 50% of cells were viable, although they exhibited a prolonged growth lag. The HHP-injured cells autolyzed in the presence of NaCl or KCl (at concentrations ≥100 mM). Superoxide dismutase slightly protected the viability of HHP-treated cells, whereas vegetative catalases had no effect. Thus, unlike HHP-injured Escherichia coli, oxidative stress only slightly affected vegetative B. subtilis subjected to HHP. Graphical abstract: Cell death and the recovery of HHP-injured Bacillus subtilis vegetative cell.