In vitro detection of diesel exhaust particles induced human lung carcinoma epithelial cells damage and the effect of resveratrol. Issue 6 (9th December 2016)

• Record Type: Journal Article
• Authors: Li, Qifei; Tang, Mingjie; Zhou, Anhong
• Is Part Of: Journal of applied toxicology. Volume 37:Issue 6(2017)
• Page Start: 747
• Page End: 757
• Publication Date: 2016-12-09
• Subjects: atomic force microscope -- Raman spectroscopy -- human lung carcinoma epithelial cells -- diesel exhaust particles -- resveratrol -- biophysical property; Toxicology -- Periodicals; Industrial toxicology -- Periodicals; Environmentally induced diseases -- Periodicals; Toxicology -- Periodicals; 615.9005
• DOI: 10.1002/jat.3423
• Languages: English
• ISSNs: 0260-437X

Abstract

Abstract: People are taking up antioxidants in their daily diet and being exposed to a potential diesel exhaust particles (DEP)‐containing environment. Thus it is important to study in vitro cellular responses when cells are exposed to DEP with or without antioxidant treatment. The investigation of DEP and resveratrol (RES) on cellular biophysical and biochemical changes is needed to better understand the mechanisms of DEP and RES in mammalian cells. A combination of two non‐invasive techniques (atomic force microscopy, AFM, and Raman spectroscopy, RM) and multimodal tools were applied to evaluate the biophysical, biochemical alterations and cytokine, membrane potential and cell cycle of cells with or without RES pretreatment to different times of DEP exposure. AFM results indicated that RES protected cells from DEP‐induced damage to cytoskeleton and cell architectures, and noted that RES treatments also attenuated DEP‐induced alterations in cell elasticity and surface adhesion force over DEP incubation time. RM monitored the changes in characteristic Raman peak intensities of DNA and protein over the DEP exposure time for both RES and non‐RES treated groups. The cytokine and chemokine changes quantified by Multiplex ELISA revealed that the inflammatory responses were enhanced with the increase in DEP exposure time and that RES enhanced the expression levels of cytokine and chemokine. This work demonstrated that significant biophysical and biochemical changes in cells might be relevant to early pathological changes induced by DEP damage. Copyright © 2016 John Wiley & Sons, Ltd. Abstract : A combination of two non‐invasive techniques (Atomic force microscopy, AFM, and Raman micro‐spectroscopy, RM) and other biological instrument tools (flow cytometry and multiplex ELISA) were applied to evaluate the biophysical, biochemical alterations and cytokine, membrane potential and cell cycle of cells with or without resveratrol (RES) pretreatment to different times of diesel exhausted particles (DEPs) exposure to better understand the mechanisms of DEP‐induced cell damages and the study of the roles of RES in DEP damaged lung cancer cells.