Synthesis of two fluorescent GTPγS molecules and their biological relevance. (3rd June 2017)

• Record Type: Journal Article
• Authors: Trans, Denise J.; Bai, Ruoli; Addison, J. Bennet; Liu, Ruiwu; Hamel, Ernest; Coleman, Matthew A.; Henderson, Paul T.
• Is Part Of: Nucleosides, nucleotides & nucleic acids. Volume 36:Number 6(2017)
• Page Start: 379
• Page End: 391
• Publication Date: 2017-06-03
• Subjects: Nucleoside and nucleotide analogs -- fluorescent nucleotide analogs -- FRET -- NMR and tubulin polymerization; Nucleosides -- Periodicals; Nucleotides -- Periodicals; Nucleic acids -- Periodicals; 572.8
• DOI: 10.1080/15257770.2016.1231320
• Languages: English
• ISSNs: 1525-7770

Abstract

ABSTRACT: Fluorescent GTP analogues are utilized for an assortment of nucleic acid and protein characterization studies. Non-hydrolysable analogues such as GTPγS offer the advantage of keeping proteins in a GTP-bound conformation due to their resistance to hydrolysis into GDP. Two novel fluorescent GTPγS molecules were developed by linking fluorescein and tetramethylrhodamine to the γ-thiophosphate of GTPγS. Chemical and biological analysis of these two compounds revealed their successful synthesis and ability to bind to the nucleotide-binding site of tubulin. These two new fluorescent non-hydrolysable nucleotides offer new possibilities for biophysical and biochemical characterization of GTP-binding proteins.