Cytotoxic and cytoprotective effects of tryptamine-4, 5-dione on neuronal cells: a double-edged sword. (4th May 2017)

• Record Type: Journal Article
• Authors: Suga, Naoko; Murakami, Akira; Nakamura, Yoshimasa; Ishisaka, Akari; Kitamoto, Noritoshi; Ito, Mikiko; Kato, Yoji
• Is Part Of: Free radical research. Volume 51:Number 5(2017:May)
• Page Start: 545
• Page End: 553
• Publication Date: 2017-05-04
• Subjects: quinone -- cytotoxicity -- neuronal cells -- quinone oxidoreductase 1 (NQO1) -- haem oxygenase 1; Free radicals (Chemistry) -- Periodicals; Antioxidants -- Periodicals; Vitamin C -- Periodicals; Vitamin E -- Periodicals; 541.224
• DOI: 10.1080/10715762.2017.1331038
• Languages: English
• ISSNs: 1071-5762

Abstract

Abstract: Serotonin (5-hydroxytryptamine) is a putative substrate for myeloperoxidase, which may convert it into the reactive quinone tryptamine-4, 5-dione (TD). In this study, we found that the viability of human SH-SY5Y neuroblastoma cells treated with 25 μM TD was increased to approximately 117%. On the other hand, the cell viability was significantly decreased by exposure to TD (150–200 μM), with an increase in intracellular reactive oxygen species (ROS). Interestingly, pre-treatment of SH-SY5Y cells with 100 μM TD prevented cell death and suppressed intracellular ROS generation evoked by the addition of hydrogen peroxide (H2 O2 ). Expression of the phase-II antioxidant enzyme NAD(P)H: quinone oxidoreductase 1 and haem oxygenase 1 were upregulated by TD at a concentration of 50–100 μM. Nuclear factor erythroid 2-related factor 2 (Nrf2), the regulator of these enzyme, was translocated from the cytosol to the nucleus by 100 μM TD. In summary, moderate concentrations of TD may increase the self-defence capacity of neuronal cells against oxidative stress.