Cryopreserved human umbilical cord versus biocellulose film for prenatal spina bifida repair in a physiologic rat model†. (16th April 2017)

• Record Type: Journal Article
• Authors: Snowise, Saul; Mann, Lovepreet; Morales, Yisel; Moise, Kenneth J.; Johnson, Anthony; Fletcher, Stephen; Grill, Raymond J.; Tseng, Scheffer C.G.; Papanna, Ramesha
• Is Part Of: Prenatal diagnosis. Volume 37:Number 5(2017)
• Page Start: 473
• Page End: 481
• Publication Date: 2017-04-16
• Subjects: Prenatal diagnosis -- Periodicals; Fetus -- Diseases -- Diagnosis -- Periodicals; Electronic journals; 618.32075
• DOI: 10.1002/pd.5035
• Languages: English
• ISSNs: 0197-3851

Abstract

Abstract: Background: Prenatal spina bifida (SB) repair with a regenerative patch may improve neurological outcomes by decreasing inflammatory scarring. Objective: This study aims to compare cryopreserved human umbilical cord (HUC) and biocellulose film (BCF) patches sutured over SB lesions for regeneration of native cells and inflammatory response. Study design: Sprague–Dawley rats were gavaged with retinoic acid (RA) on embryonic day 10 to induce SB. Hysterotomy was performed on embryonic day 20 and on HUC or BCF patches sutured over the defect. Pups were harvested 30 to 34 h later, and hematoxylin and eosin staining and Trichrome staining assessed basic cellular migration. Immunohistochemistry demonstrated the exact nature of the cellular migration. Patches and surrounding exudates were evaluated with microscopy and cells quantified. Results: Histology showed cellular migration with all HUC patches compared with none with BCF patches. Epithelial cells were noted migrating over the dorsal HUC surface, astrocytes were noted along the HUC surface adjacent to the lesion, and endothelial cells were noted within the HUC. HUC patches showed minimal inflammatory cells. Exudates surrounding the HUC patches had fewer inflammatory cells than exudates around BCF patches. Conclusion: HUC promotes cellular migration of native cells with minimal inflammatory response compared with BCF. HUC may be the superior patch material for prenatal SB repair. © 2017 John Wiley & Sons, Ltd. Abstract : What's already known about this topic? In utero spina bifida repair improves spinal cord function, but most patients have scar formation at the repair site, and neonatal outcomes are not yet optimal. A regenerative patch that would reduce inflammation and subsequent scar formation and allow for migration of native cells may improve outcomes. What does this study add? Cryopreserved human umbilical cord patches allow migration of native cells in an organized fashion and induces minimal inflammation when applied over a physiologic spina bifida lesion. Biocellulose film causes migration of inflammatory cells only.