Enzymatically biomineralized chitosan scaffolds for tissue‐engineering applications. (15th June 2015)

• Record Type: Journal Article
• Authors: Dash, Mamoni; Samal, Sangram K.; Douglas, Timothy E. L.; Schaubroeck, David; Leeuwenburgh, Sander C.; Van Der Voort, Pascal; Declercq, Heidi A.; Dubruel, Peter
• Is Part Of: Journal of tissue engineering and regenerative medicine. Volume 11:Number 5(2017)
• Page Start: 1500
• Page End: 1513
• Publication Date: 2015-06-15
• Subjects: chitosan -- enzyme -- alkaline phosphatase -- biomineralization -- scaffolds -- bone -- osteogenic -- tissue engineering; Tissue engineering -- Periodicals; Regeneration (Biology) -- Periodicals; 610.28
• DOI: 10.1002/term.2048
• Languages: English
• ISSNs: 1932-6254

Abstract

Abstract: Porous biodegradable scaffolds represent promising candidates for tissue‐engineering applications because of their capability to be preseeded with cells. We report an uncrosslinked chitosan scaffold designed with the aim of inducing and supporting enzyme‐mediated formation of apatite minerals in the absence of osteogenic growth factors. To realize this, natural enzyme alkaline phosphatase (ALP) was incorporated into uncrosslinked chitosan scaffolds. The uncrosslinked chitosan makes available amine and alcohol functionalities to enhance the biomineralization process. The physicochemical findings revealed homogeneous mineralization, with the phase structure of the formed minerals resembling that of apatite at low mineral concentrations, and similar to dicalcium phosphate dihydrate (DCPD) with increasing ALP content. The MC3T3 cell activity clearly showed that the mineralization of the chitosan scaffolds was effective in improving cellular adhesion, proliferation and colonization. Copyright © 2015 John Wiley & Sons, Ltd.