An Integrin‐Targeting RGDK‐Tagged Nanocarrier: Anticancer Efficacy of Loaded Curcumin. (2nd May 2017) - Das, Krishnendu
Abstract: Herein we report the design and development of α5 β1 integrin‐specific noncovalent RGDK–lipopeptide‐functionalized single‐walled carbon nanotubes (SWNTs) that selectively deliver the anticancer drug curcumin to tumor cells. RGDK tetrapeptide‐tagged amphiphiles were synthesized that efficiently disperse SWNTs with a suspension stability index of >80 % in cell culture media. 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide (MTT)‐ and lactate dehydrogenase (LDH)‐based cell viability assays in tumor (B16F10 melanoma) and noncancerous (NIH3T3 mouse fibroblast) cells revealed the non‐cytotoxic nature of these RGDK–lipopeptide–SWNT conjugates. Cellular uptake experiments with monoclonal antibodies against αv β3, αv β5, and α5 β1 integrins showed that these SWNT nanovectors deliver their cargo (Cy3‐labeled oligonucleotides, Cy3‐oligo) to B16F10 cells selectively via α5 β1 integrin. Notably, the nanovectors failed to deliver the Cy3‐oligo to NIH3T3 cells. The RGDK–SWNT is capable of delivering the anticancer drug curcumin to B16F10 cells more efficiently than NIH3T3 cells, leading to selective killing of B16F10 cells. Results of Annexin V binding based flow cytometry experiments are consistent with selective killing of tumor cells through the late apoptotic pathway. Biodistribution studies in melanoma (B16F10)‐bearing C57BL/6J mice showed tumor‐selective accumulation of curcumin intravenously administered via RGDK–lipopeptide–SWNT nanovectors. Abstract : Right on target : An RGDK‐tagged single‐walled nanotube (SWNT) vector was shown to selectively transport Cy3‐labeled oligonucleotides into B16F10 melanoma cells while avoiding noncancerous NIH3T3 cells. α5 β1 Integrins overexpressed at the B16F10 cell surface facilitate this internalization process. SWNTs loaded with the anticancer drug curcumin were found to selectively kill B16F10 cells more efficiently than NIH3T3 cells. Moreover, tumor‐selective accumulation of curcumin in C57BL/6J mice with B16F10 melanoma via the RGDK–lipopeptide–SWNT vector underscores its potential as an anticancer agent.