Cerebral watershed infarcts may be induced by hemodynamic changes in blood flow. (3rd June 2017)
- Record Type:
- Journal Article
- Title:
- Cerebral watershed infarcts may be induced by hemodynamic changes in blood flow. (3rd June 2017)
- Main Title:
- Cerebral watershed infarcts may be induced by hemodynamic changes in blood flow
- Authors:
- Shi, Jingfei
Meng, Ran
Konakondla, Sanjay
Ding, Yuchuan
Duan, Yunxia
Wu, Di
Wang, Bincheng
Luo, Yinghao
Ji, Xunming - Abstract:
- Abstract: Objectives: A watershed infarct is defined as an ischemic lesion at the border zones between territories of two major arteries. The pathogenesis of watershed infarcts, specifically whether they are caused by hemodynamic or embolic mechanisms, has long been debated. In this study, we aimed to examine whether watershed infarcts can be induced by altering the hemodynamic conditions in rats. Materials and Methods: In phase one, to determine the proper clamping duration for a reproducible infarct, 30 rats were equally divided into 5 subgroups and underwent bilateral common carotid artery (CCA) clamping for different durations (0.5, 1.0, 1.5, 2.0, and 3.0 hours). In phase two, to analyze the types of infarcts induced by bilateral CCA clamping, 40 rats were subjected to bilateral CCA clamping for 2 hours. As a control, 8 rats underwent all the operation procedures except bilateral CCA clamping. We performed 7.0T magnetic resonance imaging on the surviving rats on the second day to evaluate the extent of the infarcts. We further identified and examined the infarcts with brain slices stained using 2, 3, 5-triphenyltetrazolium chloride (TTC) on the third day. Results: After 2 hours of bilateral CCA clamping, cerebral infarction occurred in 42% of surviving rats (13/31). The majority of the ischemic lesions were located in watershed regions of the brain, demonstrated by both MRI and TTC staining. Conclusion: Watershed infarcts were induced through changing hemodynamicAbstract: Objectives: A watershed infarct is defined as an ischemic lesion at the border zones between territories of two major arteries. The pathogenesis of watershed infarcts, specifically whether they are caused by hemodynamic or embolic mechanisms, has long been debated. In this study, we aimed to examine whether watershed infarcts can be induced by altering the hemodynamic conditions in rats. Materials and Methods: In phase one, to determine the proper clamping duration for a reproducible infarct, 30 rats were equally divided into 5 subgroups and underwent bilateral common carotid artery (CCA) clamping for different durations (0.5, 1.0, 1.5, 2.0, and 3.0 hours). In phase two, to analyze the types of infarcts induced by bilateral CCA clamping, 40 rats were subjected to bilateral CCA clamping for 2 hours. As a control, 8 rats underwent all the operation procedures except bilateral CCA clamping. We performed 7.0T magnetic resonance imaging on the surviving rats on the second day to evaluate the extent of the infarcts. We further identified and examined the infarcts with brain slices stained using 2, 3, 5-triphenyltetrazolium chloride (TTC) on the third day. Results: After 2 hours of bilateral CCA clamping, cerebral infarction occurred in 42% of surviving rats (13/31). The majority of the ischemic lesions were located in watershed regions of the brain, demonstrated by both MRI and TTC staining. Conclusion: Watershed infarcts were induced through changing hemodynamic conditions by bilateral CCA clamping in rats. This method may lead to the development of a reliable rodent model for watershed infarcts. … (more)
- Is Part Of:
- Neurological research. Volume 39:Number 6(2017)
- Journal:
- Neurological research
- Issue:
- Volume 39:Number 6(2017)
- Issue Display:
- Volume 39, Issue 6 (2017)
- Year:
- 2017
- Volume:
- 39
- Issue:
- 6
- Issue Sort Value:
- 2017-0039-0006-0000
- Page Start:
- 538
- Page End:
- 544
- Publication Date:
- 2017-06-03
- Subjects:
- Rat -- magnetic resonance imaging -- bilateral CCA clamping -- stroke
Neurology -- Periodicals
Neurosciences -- Periodicals
616.8005 - Journal URLs:
- http://catalog.hathitrust.org/api/volumes/oclc/3983345.html ↗
http://www.ingentaconnect.com/content/maney/nres ↗
http://www.maney.co.uk/search?fwaction=show&fwid=503 ↗
http://www.tandfonline.com/toc/yner20/current ↗
http://maneypublishing.com/ ↗ - DOI:
- 10.1080/01616412.2017.1315499 ↗
- Languages:
- English
- ISSNs:
- 0161-6412
- Deposit Type:
- Legaldeposit
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- Available online (eLD content is only available in our Reading Rooms) ↗
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- British Library DSC - BLDSS-3PM
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