Plasma treated with amotosalen and ultraviolet A light retains activity for hemostasis after 5 days post‐thaw storage at 1 to 6oC. Issue 4 (1st February 2017)

• Record Type: Journal Article
• Authors: Erickson, Anna; Waldhaus, Katie; David, Tovo; Huang, Norman; Rico, Salvador; Corash, Laurence; Mufti, Nina; Benjamin, Richard J.
• Is Part Of: Transfusion. Volume 57:Issue 4(2017)
• Page Start: 997
• Page End: 1006
• Publication Date: 2017-02-01
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.13973
• Languages: English
• ISSNs: 0041-1132

Abstract

Abstract : BACKGROUND: Plasma thawed and stored at 1 to 6 ° C for up to 5 days (thawed plasma [TP]) provides rapid availability in emergencies and reduces plasma waste, but it carries risks of coagulation factor loss or activation, bacterial outgrowth, and viral contamination. We characterized changes in amotosalen/ultraviolet A (UVA) light pathogen‐reduced, fresh‐frozen plasma (FFP) and plasma frozen within 24 hours (PF24) with post‐thaw storage. STUDY DESIGN AND METHODS: Amotosalen/UVA light‐treated FFP and PF24 were thawed after approximately 3 to more than 12 months of frozen storage and held at 1 to 6 ° C for 5 days. Global assessments of coagulation and hemostatic, antithrombotic, and activation markers indicative of function were assessed. RESULTS: Day 5, thawed amotosalen/UVA light‐treated FFP and PF24 contained levels of Factors II, V, VIII, IX, X, von Willebrand factor ristocetin cofactor (vWF:RCo), fibrinogen, antithrombin III (ATIII), protein C, and protein S similar to the levels measured in Day 5 TP, as described in the Circular of Information . Thrombin generation was robust on Day 5 (amotosalen/UVA: FFP = 1866 ± 402 nM/minute; PF24 = 1800 ± 277 nM/minute). Most factor activities on Day 5, including von Willebrand factor‐cleaving protease (ADAMTS‐13), were more than 90% of Day 0 values, except for known labile Factors V and VIII and protein S. All units contained greater than 0.4 IU/mL protein S and α2 plasmin inhibitor on Day 5. Global functional indices, including thrombin‐antithrombin complexes, nonactivated thromboplastin time, and thrombin‐generation peak height, did not indicate activation of the coagulation cascade, although isolated units showed raised levels of Factor VIIa and Complement 3a. CONCLUSION: Amotosalen/UVA light‐treated FFP and PF24 demonstrated retention of procoagulant and antithrombotic activity after 5 days post‐thaw storage at 1 to 6 ° C.