Water-soluble and organic extracts of ambient PM2.5 in Tehran air: assessment of genotoxic effects on human lung epithelial cells (A549) by the Comet assay. (3rd April 2017)

• Record Type: Journal Article
• Authors: Mohseni Bandpi, A.; Eslami, A.; Shahsavani, A.; Khodagholi, F.; Aliaghaei, A.; Alinejad, A.
• Is Part Of: Toxin reviews. Volume 36:Number 2(2017)
• Page Start: 116
• Page End: 124
• Publication Date: 2017-04-03
• Subjects: Lung epithelial cells (A549) -- particulate matter PM2.5 -- physicochemical characteristics -- genotoxicity -- Comet assay; Toxicology -- Periodicals; Toxins -- Periodicals; 615.905
• DOI: 10.1080/15569543.2016.1259634
• Languages: English
• ISSNs: 1556-9543

Abstract

Abstract: The main aim of the present study is to investigate the physicochemical characterization of water-soluble extract (WS) and organic extract (OE) of PM2.5 ambient in Tehran city air in order to evaluate the genotoxicity effects and the potential attribution to these effects. The lung epithelial cells (A549) were exposed to WS and OE, while Comet assays were conducted to analyze the genotoxicity. The results show that the amount of DNA damage in WS fraction and solvent-extractable organic samples is significantly higher than the control group and the increase in concentration significantly contributes to increase in the amount of DNA damage.