Chemical genomic analysis of GPR35 signaling. Issue 5 (20th April 2017)

• Record Type: Journal Article
• Authors: Hu, Heidi (Haibei); Deng, Huayun; Ling, Shizhang; Sun, Haiyan; Kenakin, Terry; Liang, Xinmiao; Fang, Ye
• Is Part Of: Integrative biology. Volume 9:Issue 5(2017:May)
• Page Start: 451
• Page End: 463
• Publication Date: 2017-04-20
• Subjects: Biology -- Periodicals; Technology -- Periodicals; Biological systems -- Periodicals; 570.5
• DOI: 10.1039/c7ib00005g
• Languages: English
• ISSNs: 1757-9694

Abstract

Abstract : Label-free human kinome shRNA screening and DNA microarray gene expression analysis offer distinct, but complementary, views of GPR35 signaling. Abstract : GPR35, a family A orphan G protein-coupled receptor, has been implicated in inflammatory, neurological, and cardiovascular diseases. However, not much is known about the signaling and functions of GPR35. We performed a label-free kinome short hairpin RNA screen and identified a putative signaling network of GPR35 in HT-29 cells, some of which was validated using gene expression, biochemical and cellular assays. The results showed that GPR35 induced hypoxia-inducible factor 1α, and was involved in synaptic transmission, sensory perception, the immune system, and morphogenetic processes. Collectively, our data suggest that GPR35 may play an important role in response to hypoxic stress and be a potential target for the treatment of inflammatory, cardiovascular, and neurological disorders.