Application of combination bubble liposomal amphotericin B and sonication has the dramatic effect on oral candidiasis. Issue 3 (May 2017)

• Record Type: Journal Article
• Authors: Yamamoto, M.; Iwanaga, K.; Okinaga, T.; Ariyoshi, W.; Tominaga, K.; Nishihara, T.
• Is Part Of: Journal of oral and maxillofacial surgery, medicine, and pathology. Volume 29:Issue 3(2017)
• Page Start: 193
• Page End: 197
• Publication Date: 2017-05
• Subjects: Liposomal amphotericin B -- Candida albicans -- Bubble liposome -- Drug delivery -- Ultrasonication; Mouth -- Surgery -- Periodicals; Face -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Oral medicine -- Periodicals; Mouth -- Diseases -- Pathogenesis -- Periodicals; Surgery, Oral -- Periodicals; Oral Medicine -- Periodicals; Pathology, Oral -- Periodicals; Face -- Surgery; Maxilla -- Surgery; Mouth -- Diseases -- Pathogenesis; Mouth -- Surgery; Oral medicine; Electronic journals -- Sciences; Electronic journals -- Medicine; Periodicals; 617.522059
• DOI: 10.1016/j.ajoms.2016.11.004
• Languages: English
• ISSNs: 2212-5566

Abstract

Abstract: Objective: We previously reported that ultrasound-mediated destruction of microbubbles might be an innovative non-invasive drug delivery system. AmBisome is a single bilayer liposomal drug delivery system consisting of unilamellar bilayer liposomes with amphotericin B, an antifungal drug, intercalated within the membrane. In the present study, we developed a novel fungicidal drug delivery system using ultrasound and bubble liposomal amphotericin B prepared by enclosing perfluoropropane gas within AmBisome. Methods: Candida albicans growth inhibition was determined by measuring optical density (OD), counting the number of colony forming units (CFU), and measuring the colony diameters. Result: Treatment with bubble liposomal amphotericin B and ultrasound significantly reduced the OD, CFU and diameter of colonies of C. albicans compared with ultrasound alone, liposomal amphotericin B alone, bubble liposomal amphotericin B alone, liposomal amphotericin B with ultrasound. Conclusion: These results indicate that treatment with bubble liposomal amphotericin B with ultrasound efficiently inhibited the growth of C. albicans, suggesting this novel approach may have a beneficial effect on the treatment of oral candidal disease.