DNA-friendly Cu(ii)/TEMPO-catalyzed 5-hydroxymethylcytosine-specific oxidation. Issue 42 (11th May 2017)

• Record Type: Journal Article
• Authors: Matsushita, Taku; Moriyama, Yuya; Nagae, Genta; Aburatani, Hiroyuki; Okamoto, Akimitsu
• Is Part Of: Chemical communications. Volume 53:Issue 42(2017)
• Page Start: 5756
• Page End: 5759
• Publication Date: 2017-05-11
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc02814h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : An allyl alcohol-selective oxidant Cu(ii )/TEMPO complex makes it possible to specifically detect 5-hydroxymethylcytosine in the human brain genome using a DNA sequence analyzer after a very easy pretreatment. Abstract : The mixture of copper(ii ) perchlorate and 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mildly and specifically oxidized 5-hydroxymethylcytosine to yield 5-formylcytosine because of its allyl alcohol specificity. The genomic DNA oxidized at 5-hydroxymethylcytosine by the copper(ii )/TEMPO oxidation was efficiently analyzed using various practical methods: DNA cleavage by piperidine, specific functionalization by hydrazone formation, and genome-wide high-resolution microarray.