Electroacupuncture preconditioning and postconditioning inhibit apoptosis and neuroinflammation induced by spinal cord ischemia reperfusion injury through enhancing autophagy in rats. (6th March 2017)

• Record Type: Journal Article
• Authors: Fang, Bo; Qin, Meiman; Li, Yun; Li, Xiaoqian; Tan, Wenfei; Zhang, Ying; Ma, Hong
• Is Part Of: Neuroscience letters. Volume 642(2017)
• Page Start: 136
• Page End: 141
• Publication Date: 2017-03-06
• Subjects: EA electroacupuncture -- I/R ischemia reperfusion -- BBB Basso-Beattie-Bresnahan -- TUNEL terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling -- TNF-α tumor necrosis factor-α -- IL-1β interleukin-1β -- MMP-9 matrix metalloproteinase-9 -- ELISA enzyme-linked immunosorbent assay -- 3-MA 3-methyladenine -- PBS phosphate buffer saline -- DAPI 4′, 6-diamidino-2-phenylindole -- LC3 microtubule-associated protein light chain 3 -- HRP horseradish peroxidase; Electroacupuncture -- Autophagy -- Spinal cord ischemia reperfusion injury -- Apoptosis -- Neuroinflammation; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2017.02.010
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Electroacupuncture preconditioning and postconditioning upregulated autophagy after spinal cord I/R injury. Electroacupuncture preconditioning and postconditioning improved neurological function recovery. Electroacupuncture preconditioning and postconditioning inhibited apoptosis through enhancing autophagy. Electroacupuncture preconditioning and postconditioning inhibited inflammation through enhancing autophagy. Abstract: Electroacupuncture (EA) has beneficial effects on spinal cord ischemia reperfusion (I/R) injury, but the underlying mechanisms are not fully understood. This study aimed to investigate the role of autophagy in the protection of EA preconditioning and postconditioning against spinal cord I/R injury. For this, spinal cord I/R injury was induced by 14 min occlusion of the aortic arch, and rats were treated with EA for 20 min before or after the surgery. The expression of autophagy components, light chain 3 and Beclin 1, was assessed by Western blot. The hind-limb motor function was assessed using the Basso-Beattie-Bresnahan (BBB) criteria, and motor neurons in the ventral gray matter were counted by histological examination. The apoptosis of neurocyte was assessed by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay. The expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and matrix metalloproteinase-9 (MMP-9) was also measured using Western blot or enzyme-linked immunosorbent assay (ELISA). Either EA preconditioning or postconditioning enhanced autophagy, and minimized the neuromotor dysfunction and histopathological deficits after spinal cord I/R injury. In addition, EA suppressed I/R-induced apoptosis and increased in the expression of TNF-α, IL-1β, and MMP-9. In contrast, the autophagic inhibitor (3-methyladenine, 3-MA) inhibited the neuroprotective effects of EA. Moreover, 3-MA increased the apoptosis and the expression of TNF-α, IL-1β, and MMP-9. In summary, these findings suggested that EA preconditioning and postconditioning could alleviate spinal cord I/R injury, which was partly mediated by autophagy upregulation-induced inhibition of apoptosis and neuroinflammation.