Anoctamin2 (TMEM16B) forms the Ca2+-activated Cl− channel in the retinal pigment epithelium. (January 2017)

• Record Type: Journal Article
• Authors: Keckeis, Susanne; Reichhart, Nadine; Roubeix, Christophe; Strauß, Olaf
• Is Part Of: Experimental eye research. Volume 154(2017)
• Page Start: 139
• Page End: 150
• Publication Date: 2017-01
• Subjects: Chloride -- Ca2+-dependent chloride channels -- anoctamin2 -- Ano2 -- ATP -- RPE; Ophthalmology -- Periodicals; Eye -- Periodicals; Œil -- Périodiques; Ophthalmology; Periodicals; Electronic journals; 612.8405
• DOI: 10.1016/j.exer.2016.12.003
• Languages: English
• ISSNs: 0014-4835

Abstract

Abstract: Chloride channels (Cl channels) play an essential role for the retinal pigment epithelium (RPE). They provide a plasma membrane conductance for Cl − important for transepithelial transport and volume regulation. Ca 2+ -dependent chloride channels (CaCC) in the RPE were found to adapt Cl − transport to specific needs by increasing intracellular free Ca 2+ . Although a variety of Cl channels have been identified in the RPE, the molecular identity of the CaCC remains controversial. Sagittal sections of mouse retina were stained against anoctamin2 (Ano2) and analyzed by confocal microscopy. Membrane currents from ARPE-19 cells and primary murine RPE cells were recorded in the whole-cell configuration of the patch-clamp technique. Expression of Ano2 was assessed via immunocytochemistry, PCR and western-blot and down-regulated via siRNA approaches. In the mouse retina, Ano2 was found in the basolateral membrane of the RPE. In primary mouse RPE cells, Ano2 was localized predominantly in the cell membrane. Ano2 mRNA and protein were also detected in rat and primate RPE as well as ARPE-19 cells. Whole-cell currents were elicited by increasing intracellular free Ca 2+ via ATP application. These currents were identified as Cl − currents by their reversal potential and blocker sensitivity. Knock-down of Ano2 by siRNA decreased both the Ca 2+ dependent chloride conductance and protein expression of Ano2. The biophysical and pharmacological properties of CaCC in ARPE-19 and primary mouse RPE cells resemble those described in previous publications using RPE cells from different species. The siRNA knock-down suggests that Ano2 contributes to Ca 2+ -dependent chloride conductance in the RPE. Graphical abstract: Highlights: Ano2 is expressed in the basolateral membrane of the RPE. ATP application elicits Ca 2+ -dependent Cl − conductance in RPE cells that is reduced by siAno2. Ano2 contributes to Ca 2+ -dependent Cl − conductance in both ARPE-19 and primary mouse RPE cells.