Development and validation of an ultraviolet–visible spectrophotometric method for determination of phenylethyl resorcinol in new topical nanoemulsions. (10th March 2017)

• Record Type: Journal Article
• Authors: Hong, L.; Han, D.; Li, M.‐X.; Zhang, P.; Liu, C.‐G.
• Is Part Of: International journal of cosmetic science. Volume 39:Number 3(2017)
• Page Start: 337
• Page End: 343
• Publication Date: 2017-03-10
• Subjects: chemical analysis -- emulsions -- phenylethyl resorcinol -- spectroscopy -- validation; Cosmetics -- Periodicals; 668.5505
• DOI: 10.1111/ics.12381
• Languages: English
• ISSNs: 0142-5463

Abstract

Abstract: Objective: To develop a rapid, simple and efficient ultraviolet–visible (UV–vis) spectrophotometric method for the quantification of phenylethyl resorcinol (PR), a potent skin‐lightening agent, incorporated into new topical nanoemulsions, and to validate this method according to International Commission for Harmonization (ICH) guidelines. Methods: UV–vis spectrophotometric method for quantification of PR in new topical nanoemulsions was developed and validated in terms of specificity, linearity, sensitivity, precision and accuracy. The method was applied to determine PR content (extraction recoveries) in the nanoemulsions and entrapment efficiencies. Results: The calibration curve for PR was linear in the range of 10–60 μ g mL −1, with a determination coefficient ( r 2 ) which is higher than 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) were 1.55 and 4.69 μ g mL −1, respectively. The method was shown to be specific, precise [intraday, interday and reproducibility levels relative standard deviation < 3%] and accurate (between 91.90 ± 1.28% and 104.73 ± 0.60%). The extraction recoveries of PR in nanoemulsions were 97.07%, 96.64% and 103.54% at concentrations of 3, 6 and 9 mg mL −1, respectively. The entrapment efficiencies were nearly 100%, which agrees with the oil core location of PR in nanoemulsions. Conclusion: This developed method was confirmed to be rapid, simple, cost‐effective, specific, precise, accurate and suitable for determination of PR content in nanoemulsions and entrapment efficiencies. Abstract : A rapid, simple and efficient ultraviolet–visible (UV–vis) spectrophotometric method has been developed for the quantification of phenylethyl resorcinol (PR), a potent skin‐lightening agent, in new topical nanoemulsions. This method was validated in terms of specificity, linearity, sensitivity, precision and accuracy according to International Commission for Harmonization (ICH) guidelines. The method was successfully applied in the determination of PR content in nanoemulsions and entrapment efficiencies.