Detection of l-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates. Issue 10 (15th May 2017)

• Record Type: Journal Article
• Authors: Cellier, Marie; James, Arthur L.; Orenga, Sylvain; Perry, John D.; Rasul, Ari K.; Stanforth, Stephen P.
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 27:Issue 10(2017)
• Page Start: 2102
• Page End: 2106
• Publication Date: 2017-05-15
• Subjects: Chromogenic enzyme substrates -- l-Alanylaminopeptidase detection -- Microorganisms; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2017.03.080
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detectingl -alanylaminopeptidase activity in microorganisms. An Alizarin-based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions.