Impact of Dye‐Protein Interaction and Silver Nanoparticles on Rose Bengal Photophysical Behavior and Protein Photocrosslinking1. (17th August 2013)

• Record Type: Journal Article
• Authors: Simpson, Madeline J.; Poblete, Horacio; Griffith, May; Alarcon, Emilio I.; Scaiano, Juan C.
• Is Part Of: Photochemistry and photobiology. Volume 89:Number 6(2013:Nov./Dec.)
• Page Start: 1433
• Page End: 1441
• Publication Date: 2013-08-17
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.12119
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: The role of recombinant Type‐I human collagen in the free form or forming AgNP@collagen on the photophysical and photochemical behavior of rose Bengal was analyzed. The formation of dye aggregates on the protein surface was experimentally observed and corroborated by docking calculations. The formation of such aggregates is believed to change the main oxidative mechanism from Type‐II (singlet oxygen) to Type‐I (free radical) photosensitization. Remarkably, the presence of AgNP in the form of AgNP@collagen altered the dynamics of dye triplet deactivation, effectively preventing the dye degradation and reducing the extent of protein crosslinked. Both crosslinked rHC and AgNP@collagen were able to support fibroblasts proliferation, but only the material containing silver was resistant to S. epidermidis infection. Abstract : "Rose Bengal binds up to 10 molecules per Type‐I collagen molecule in aqueous solutions. Upon binding of rose Bengal the photophysical and photochemical properties of the dye are strongly affected. The presence of silver nanoparticles in the near vicinity of the protein further modifies the photophysical properties of the dye".