The role of miR-17-92 cluster in the expression of tumor suppressor genes in unrestricted somatic stem cells. (March 2017)

• Record Type: Journal Article
• Authors: Tavakoli, Rezvan; Vakilian, Saeid; Langroudi, Lida; Arefian, Ehsan; Sahmani, Mehdi; Soleimani, Masoud; Jamshidi-Adegani, Fatemeh
• Is Part Of: Biologicals. Volume 46(2017)
• Page Start: 143
• Page End: 147
• Publication Date: 2017-03
• Subjects: Tumor suppressor gene -- Tumorigenesis -- microRNAs -- miR-17-92 cluster; Biological products -- Standards -- Periodicals; Biological Products -- Periodicals; Biological Products -- standards -- Periodicals; Produits biologiques -- Normes -- Périodiques; Biological products -- Standards; Periodicals; 615.37
• DOI: 10.1016/j.biologicals.2017.02.006
• Languages: English
• ISSNs: 1045-1056

Abstract

Abstract: The miR-17-92 cluster consisted of seven miRNAs (mir-17-5p, -17-3p, -18a, -19a, -20a, -19b-1, and -92a-1). Previous studies have shown this cluster has been over-expressed in several cancers. The aim of this study was to evaluate the over-expression impacts of miR-17-92 on stem cells. In the current work, the effect of miR-17-92 cluster which was cloned in Lentiviral vector has been investigated on unrestricted somatic stem cells (USSCs). Tumor suppressor genes (p53, p15, RBL1, SMAD2, SMAD4, and MAPK-1) expression, especially p53, was considerably reduced. These data show the potential of miR-17-92 for oncogenesis regulation in stem cells. In conclusion, the role of miR-17-92 in USSCs may provide a better understanding of its function in tumorigenesis and for the possible use in cell therapy of the anti-mir-17-92 cluster.