Precisely Regulated and Efficient Locking of Linear Peptides into Stable Multicyclic Topologies through a One‐Pot Reaction. Issue 16 (27th February 2017)

• Record Type: Journal Article
• Authors: Liu, Weidong; Zheng, Yiwu; Kong, Xudong; Heinis, Christian; Zhao, Yibing; Wu, Chuanliu
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 16(2017)
• Page Start: 4458
• Page End: 4463
• Publication Date: 2017-02-27
• Subjects: cyclization -- cysteine -- penicillamine -- peptides -- thioether bonds; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201610942
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: We report the discovery of a small phenyl molecule with four isosteric thiolate‐reactive groups of sequentially varied reactivity. This molecule was exploited in combination with cysteine/penicillamine thiolates of different nucleophilic reactivity for precisely regulated and efficient locking (PROP‐locking) of linear peptides into multicyclic topologies through a one‐pot reaction. The PROP‐locking relies on multistep and sequential thiolate/fluorine nucleophilic substitutions, which is not only rapid but highly specific, thus enabling rapid locking of peptides with high amino acid diversities without protecting groups. Several tricyclic peptide templates and bioactive peptides were designed and synthesized using the PROP‐locking strategy. We believe that tricyclic peptides precisely locked through stable thioether bonds should be promising structurally constrained scaffolds for developing potential therapeutics and target ligands. Abstract : Bioactive tricyclic peptide ligands : A small phenyl molecule with four isosteric thiolate‐reactive groups of varied reactivity was discovered. This molecule was used in combination with cysteine/penicillamine thiolate groups of different nucleophilic reactivity for precisely regulated and efficient locking of a linear peptide into multicyclic topologies through a one‐pot reaction.