Molecular characteristics, biofilm-forming abilities, and quorum sensing molecules in Vibrio parahaemolyticus strains isolated from marine and clinical environments in Korea. Issue 5 (28th May 2017)

• Record Type: Journal Article
• Authors: Mizan, Md. Furkanur Rahaman; Bang, Hyeon-Jo; Sadekuzzaman, Mohammad; Lee, Nari; Kim, Tae-Jo; Ha, Sang-Do
• Is Part Of: Biofouling. Volume 33:Issue 5(2017)
• Page Start: 369
• Page End: 378
• Publication Date: 2017-05-28
• Subjects: Vibrio parahaemolyticus -- REP-PCR -- AI-2 -- HPLC-FLD -- UltraFast LabChip Real-Time PCR; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2017.1316840
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: Vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. In this study, an UltraFast LabChip Real-Time PCR assay was evaluated for rapid detection and quantification of pathogenic V. parahaemolyticus isolates. Escherichia coli and Vibrio harveyi were used as negative controls. Twenty-six tdh -positive, biofilm-producing V. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). REP-PCR analysis showed that the majority of the V. parahaemolyticus isolates originated from seafood and that clinical specimens formed two major clusters at 92.8% and 32% similarity levels. The presence and quantification of Autoinducer-2 was carried out using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) after derivatization of Autoinducer-2 with 2, 3-diaminonaphthalene. The presence of tdh -positive V. parahaemolyticus in marine samples highlights the need for constant environmental monitoring to protect public health.