A highly selective and sensitive fluorescent probe for Cu2+ based on a novel naphthalimide–rhodamine platform and its application in live cell imaging. Issue 18 (24th April 2017)

• Record Type: Journal Article
• Authors: Liu, Chang; Jiao, Xiaojie; He, Song; Zhao, Liancheng; Zeng, Xianshun
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 18(2017)
• Page Start: 3947
• Page End: 3954
• Publication Date: 2017-04-24
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c7ob00538e
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A bifluorophore-based ratiometric fluorescent probe has been developed. The probe is cell-permeable and can be used as a FRET-based ratiometric fluorescent probe for lysosomal Cu 2+ imaging in living cells. Abstract : Copper plays important roles in a variety of fundamental physiological processes. At the cell organelle level, aberrant copper homeostasis in lysosomes can lead to various serious diseases. Herein, a bifluorophore-based, lysosome-targetable Cu 2+ -selective ratiometric fluorescent probe (V ) has been synthesized by reasonable design. The probeV shows high selectivity toward Cu 2+ ions over other cations and exhibits high sensitivity (1.45 nM) for the detection of Cu 2+ ions. Meanwhile, the probe is cell permeable and suitable for ratiometric visualization of lysosomal Cu 2+ in the living cell.