Accumulation, elimination, sequestration, and genetic variation of lead (Pb2+) loads within and between generations of Drosophila melanogaster. (August 2017)

• Record Type: Journal Article
• Authors: Peterson, Elizabeth K.; Wilson, Diane T.; Possidente, Bernard; McDaniel, Phillip; Morley, Eric J.; Possidente, Debra; Hollocher, Kurt T.; Ruden, Douglas M.; Hirsch, Helmut V.B.
• Is Part Of: Chemosphere. Volume 181(2017)
• Page Start: 368
• Page End: 375
• Publication Date: 2017-08
• Subjects: Pb load -- Accumulation -- Sequestration -- Elimination -- Genetic variation; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.04.091
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: We examined accumulation, sequestration, elimination, and genetic variation for lead (Pb) loads within and between generations of Drosophila melanogaster . Flies were reared in control or leaded medium at various doses and tested for their Pb loads at different stages of development (larvae, eclosion, newly-eclosed adults, and mature adults). Pb loads were tested using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). We found that D. melanogaster readily accumulated Pb throughout their lifespan and the levels of accumulation increased with Pb exposure in the medium. Wandering third-instar larvae accumulated more Pb than mature adults; this phenomenon may be due to elimination of Pb in the pupal cases during eclosion and/or depuration in adults post-eclosion. The accumulated Pb in mature adults was not transferred to F1 mature adult offspring. Using a set of recombinant inbred strains, we identified a quantitative trait locus for adult Pb loads and found that genetic variation accounted for 34% of the variance in Pb load. We concluded that D. melanogaster is a useful model organism for evaluating changes in Pb loads during development, as well as between generations. Furthermore, we found that genetic factors can influence Pb loads; this provides an essential foundation for evaluating phenotypic variation induced by the toxic effects of Pb. Highlights: D. melanogaster accumulated Pb throughout their life. Levels of accumulation increased with Pb dose. Wandering third-instar larvae accumulated more Pb than mature adults. The accumulated Pb in mature adults was not transferred to F1 mature adult offspring. We found a quantitative trait locus for adult Pb loads. Genetic variation accounted for 34% of the variance in Pb load.