Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca2+‐regulated Photoproteins of Different Organisms1. (16th December 2016)

• Record Type: Journal Article
• Authors: Eremeeva, Elena V.; Bartsev, Sergey I.; van Berkel, Willem J. H.; Vysotski, Eugene S.
• Is Part Of: Photochemistry and photobiology. Volume 93:Number 2(2017)
• Page Start: 495
• Page End: 502
• Publication Date: 2016-12-16
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.12664
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: Upon binding their metal ion cofactors, Ca 2+ ‐regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca 2+ ‐regulated photoproteins—aequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculata —demonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca 2+ ‐regulated photoproteins. Abstract : Bioluminescence of a great number of marine organisms, mostly coelenterates, is due to the presence of Ca 2+ ‐regulated photoproteins demonstrating the same bioluminescent kinetics pattern. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family using five recombinant hydromedusan Ca 2+ ‐regulated photoproteins—aequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculata . For the first time, we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca 2+ ‐regulated photoproteins.