Gastric microbiota features associated with cancer risk factors and clinical outcomes: A pilot study in gastric cardia cancer patients from Shanxi, China. Issue 1 (3rd April 2017)

• Record Type: Journal Article
• Authors: Yu, Guoqin; Hu, Nan; Wang, Lemin; Wang, Chaoyu; Han, Xiao‐You; Humphry, Mike; Ravel, Jacques; Abnet, Christian C.; Taylor, Philip R.; Goldstein, Alisa M.
• Is Part Of: International journal of cancer. Volume 141:Issue 1(2017:Jul. 01)
• Page Start: 45
• Page End: 51
• Publication Date: 2017-04-03
• Subjects: H. pylori -- 16S rRNA gene -- KEGG modules -- microbiome -- gastric cancer; Cancer -- Periodicals; Cancer -- Prevention -- Periodicals; 616.994
• DOI: 10.1002/ijc.30700
• Languages: English
• ISSNs: 0020-7136

Abstract

Abstract : Little is known about the link between gastric microbiota and the epidemiology of gastric cancer. In order to determine the epidemiologic and clinical relevance of gastric microbiota, we used 16 S ribosomal RNA gene sequencing analysis to characterize the composition and structure of the gastric microbial community of 80 paired samples (non‐malignant and matched tumor tissues) from gastric cardia adenocarcinoma (GCA) patients in Shanxi, China. We also used PICRUSt to predict microbial functional profiles. Compared to patients without family history of upper gastrointestinal (UGI) cancer in the non‐malignant gastric tissue microbiota, patients with family history of UGI cancer had higher Helicobacter pylori ( Hp) relative abundance (median: 0.83 vs . 0.38, p = 0.01) and lower alpha diversity (median observed species: 51 vs . 85, p = 0.01). Patients with higher ( vs . lower) tumor grade had higher Hp relative abundance (0.73 vs . 0.18, p = 0.03), lower alpha diversity (observed species, 66 vs . 89, p = 0.01), altered beta diversity (weighted UniFrac, p = 0.002) and significant alterations in relative abundance of five KEGG functional modules in non‐malignant gastric tissue microbiota. Patients without metastases had higher relative abundance of Lactobacillales than patients with metastases (0.05 vs . 0.01, p = 0.04) in non‐malignant gastric tissue microbiota. These associations were observed in non‐malignant tissues but not in tumor tissues. In conclusion, this study showed a link of gastric microbiota to a major gastric cancer risk factor and clinical features in GCA patients from Shanxi, China. Studies with both healthy controls and gastric cardia and noncardia cancer cases across different populations are needed to further examine the association between gastric cancer and the microbiota. Abstract : What's new? Studies of the diverse gastric microbiota in humans are scarce and limited. And while chronic infection by Helicobacter pylori is the major risk factor of gastric cardia adenocarcinoma in Eastern populations, the role of non‐ Hp gastric microbiota in human health is unclear. This is the first study to link gastric microbiota to known cancer risk factors and clinical outcomes in gastric cardia cancer patients from China. H. pylori relative abundance and microbiota diversity differed between patients with and without a family history of upper gastrointestinal tract cancer. The study also revealed associations between tumor grade, metastasis and gastric microbiota features.