Structure of the O-specific polysaccharide from a marine bacterium Cellulophaga algicola. (18th April 2017)

• Record Type: Journal Article
• Authors: Tomshich, Svetlana V.; Kokoulin, Maxim S.; Kalinovsky, Anatoliy I.; Nedashkovskaya, Ol'ga I.; Komandrova, Nadezhda A.
• Is Part Of: Carbohydrate research. Volume 443/444(2017)
• Page Start: 68
• Page End: 72
• Publication Date: 2017-04-18
• Subjects: O-specific polysaccharide -- Cellulophaga algicola -- Bacterial polysaccharide structure -- Marine bacterium; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2017.03.017
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: The O-polysaccharide was isolated from the lipopolysaccharide of Cellulophaga algicola and studied by chemical analyses along with 1 H and 13 C NMR spectroscopy, including 2D 1 H, 1 H COSY, TOCSY, ROESY, 1 Н, 13 С HSQC, HMBC experiments. It was found that the polysaccharide is built up of branched pentasaccharide repeating units, containing D-mannose (Man), D-glucuronic acid (GlcA), N -acetyl-D-glucosamine (GlcNAc), two L-rhamnose (Rha) residues and O-acetyl groups in a non-stoichiometric amount and has the following structure: Graphical abstract: Highlights: The O-polysaccharide was isolated from a marine bacterium Cellulophaga algicola. Structure of the polysaccharide was established by NMR and chemical approaches. The polysaccharide is built up of branched pentasaccharide repeating units.