MALDI imaging on large‐scale tissue microarrays identifies molecular features associated with tumour phenotype in oesophageal cancer. Issue 4 (16th July 2013)

• Record Type: Journal Article
• Authors: Quaas, Alexander; Bahar, Ahmad Soliaman; von Loga, Katharina; Seddiqi, Ahmad Shoaib; Singer, Julius Magnus; Omidi, Maryam; Kraus, Olga; Kwiatkowski, Marcel; Trusch, Maria; Minner, Sarah; Burandt, Eike; Stahl, Phillip; Wilczak, Waldemar; Wurlitzer, Marcus; Simon, Ronald; Sauter, Guido; Marx, Andreas; Schlüter, Hartmut
• Is Part Of: Histopathology. Volume 63:Issue 4(2014)
• Page Start: 455
• Page End: 462
• Publication Date: 2013-07-16
• Subjects: MALDI mass spectrometry imaging -- oesophageal cancer -- tissue microarray; Histology, Pathological -- Periodicals; 611.018
• DOI: 10.1111/his.12193
• Languages: English
• ISSNs: 0309-0167

Abstract

Abstract : Aims: Matrix‐assisted laser desorption/ionisation mass spectrometry imaging (MALDI‐MSI) and tissue microarray (TMA) technologies were jointly utilized to search for molecular features associated with clinicopathological parameters in oesophageal cancer. Methods and results: Two TMAs from formalin‐fixed tissue samples, including 300 adenocarcinomas and 177 squamous cell carcinomas with clinical follow‐up data, were analysed. MALDI‐MSI analysis revealed 72 distinct mass per charge ( m / z ) signals associated with tumour cells, 48 of which were found in squamous cell carcinomas only, and 12 of which were specific for adenocarcinomas. In adenocarcinomas, six signals were linked to early‐stage (pT1–T2) tumours (two signals) and the presence (one signal) or absence (three signals) of lymph node metastasis. In squamous cell carcinomas, 24 signals were strongly linked to different phenotypic features, including tumour stage (four signals), histological grade (four signals), and lymph node metastasis (three signals). Conclusions: The high number of m / z signals that were found to be significantly linked to one or more phenotypic features of oesophageal cancer highlights the power of MALDI‐MSI in the analysis of high‐density TMAs. The data also emphasise substantial biological differences between adenocarcinomas and squamous cell carcinomas.