Quarantine versus pathogen‐reduced plasma‐coagulation factor content and rotational thromboelastometry coagulation. Issue 3 (27th November 2016)

• Record Type: Journal Article
• Authors: Theusinger, Oliver M.; Goslings, David; Studt, Jan‐Dirk; Brand‐Staufer, Brigitte; Seifert, Burkhardt; Spahn, Donat R.; Frey, Beat M.
• Is Part Of: Transfusion. Volume 57:Issue 3(2017)
• Page Start: 637
• Page End: 645
• Publication Date: 2016-11-27
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.13935
• Languages: English
• ISSNs: 0041-1132

Abstract

Abstract : BACKGROUND: Different types of fresh‐frozen plasma (FFP) exist, and the concentrations of plasma proteins vary between individuals and blood groups. Furthermore, processing may also influence the content. Quarantine‐stored plasma (qFFP) and plasma that was pathogen‐reduced using blood‐safety (Intercept) technology (piFFP) were analyzed regarding procoagulant and anticoagulant hemostasis proteins, including endogenous thrombin (thrombin‐generation) potential (ETP). MATERIALS AND METHODS: Thirty‐five samples of each type of FFP were analyzed using only male Blood Group O donors. FFP units were stored frozen for comparable periods of time before plasma protein content was assessed. Once the units were thawed, all tests were completed within 4 hours. The results are presented as means ± standard deviations or as median (minimum; maximum) and were compared using independent‐sample t tests (significance, p < 0.01). RESULTS: Significantly higher concentrations of adintegrin‐like and metalloprotease with thrombospondin type‐13 motifs (ADAMTS13), fibrinogen, Factor (F)V, FVIII, FXIII, protein S, protein S activity, antithrombin, microvesicle (<900 nm), and α2 antiplasmin were observed in qFFP. The variability of factors was significantly lower in piFFP. Tissue factor (TF) at 1 picomolar (pM) exhibited significantly longer lag time, a lower peak, lower ETP, and a lower velocity index in qFFP compared with piFFP. In TF at 5 pM, significant differences in lag time (longer in qFFP), velocity index (lower in qFFP), and peak (lower in qFFP) were observed. Rotational thromboelastometry revealed a significantly longer (p = 0.002) clot‐formation time with intrinsic thromboelastometry for piFFP and a significantly shorter clotting time (p = 0.004) with thromboelastometry fibrinogen testing for piFFP. CONCLUSION: Pathogen reduction reduces procoagulant and anticoagulant coagulation factors as well as variability. A thrombin‐generation assay showed no reduced ETP and no supraphysiological thrombin generation. None of the FFP preparations is likely to be effective for treating fibrinogen deficiency.