IL-1β directly suppress ghrelin mRNA expression in ghrelin-producing cells. (15th May 2017)

• Record Type: Journal Article
• Authors: Bando, Mika; Iwakura, Hiroshi; Ueda, Yoko; Ariyasu, Hiroyuki; Inaba, Hidefumi; Furukawa, Yasushi; Furuta, Hiroto; Nishi, Masahiro; Akamizu, Takashi
• Is Part Of: Molecular and cellular endocrinology. Volume 447(2017)
• Page Start: 45
• Page End: 51
• Publication Date: 2017-05-15
• Subjects: Ghrelin -- IL-1β; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2017.02.032
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: In animal models, ghrelin production is suppressed by LPS administration. To elucidate the detailed molecular mechanisms involved in the phenomenon, we investigated the effects of LPS and LPS-inducible cytokines, including TNF-α, IL-1β, and IL-6, on the expression of ghrelin in the ghrelin-producing cell line MGN3-1. These cells expressed IL-1R, and IL-1β significantly suppressed ghrelin mRNA levels. The suppressive effects of IL-1β were attenuated by knockdown of IKKβ, suggesting the involvement of the NF-κB pathway. These results suggested that IL-1β is a major regulator of ghrelin expression during inflammatory processes. Highlights: IL-1β significantly suppresses ghrelin mRNA levels in MGN3-1 cells. Ghrelin-producing cells express IL-1R. The NF-κB pathway is involved in IL-1β–induced suppression of ghrelin.