17β-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. (5th May 2017)

• Record Type: Journal Article
• Authors: Zafirovic, Sonja; Obradovic, Milan; Sudar-Milovanovic, Emina; Jovanovic, Aleksandra; Stanimirovic, Julijana; Stewart, Alan J.; Pitt, Samantha J.; Isenovic, Esma R.
• Is Part Of: Molecular and cellular endocrinology. Volume 446(2017)
• Page Start: 12
• Page End: 20
• Publication Date: 2017-05-05
• Subjects: Estradiol -- Obesity -- Myocardial metabolism -- Cardiovascular diseases -- Inducible nitric oxide synthase; Akt protein kinase B -- AS160 Akt substrate of 160 kDa -- CD36 fatty acid transporters -- E2 estradiol -- FFA free fatty acids -- GLUT glucose transporters -- HF high fat -- IR insulin resistance -- IRS1 insulin receptor substrate-1 -- L-arg L-arginine -- LDM low density microsomes -- NO nitric oxide -- PM plasma membrane -- NOS NO synthase -- iNOS inducible NOS -- NFkB nuclear factor kappa-B -- OVX ovariectomized -- PI3K phosphatidylinositol 3-kinase; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2017.02.001
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: The aim of this study was to investigate the in vivo effects of 17β-estradiol (E2 ) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p < 0.05), L-arginine (p < 0.01), iNOS mRNA (p < 0.01), and protein (p < 0.05) levels and translocation of the FFA transporter (CD36) (p < 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr 308 (p < 0.05) and translocation of the glucose transporter GLUT4 (p < 0.05) to the PM increased after E2 treatment in HF rats. Our results indicate that E2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats. Graphical abstract: Highlights: Estradiol treatment influences myocardial glucose and free fatty acid metabolism in rats fed a high fat (HF) diet. In vivo estradiol treatment impacts on cardiac iNOS expression/activity in HF fed rats. Estradiol acts via Akt to attenuate the detrimental effects of cardiac iNOS in HF fed rats.