A modified vitrification method reduces spindle and chromosome abnormalities. (4th May 2017)

• Record Type: Journal Article
• Authors: Liu, Minghui; Zhou, Wenhui; Chu, Dapeng; Fu, Lei; Sha, Wei; Liu, Shan; Li, Yuan
• Is Part Of: Systems biology in reproductive medicine. Volume 63:Number 3(2017:Jun.)
• Page Start: 199
• Page End: 205
• Publication Date: 2017-05-04
• Subjects: Human oocyte -- pre-equilibrium temperature -- vitrification; Systems biology -- Periodicals; Andrology -- Periodicals; Generative organs, Male -- Diseases -- Periodicals; Biological systems -- Periodicals; Reproductive health -- Periodicals; Human reproduction -- Periodicals; 612.61
• DOI: 10.1080/19396368.2017.1285370
• Languages: English
• ISSNs: 1939-6368

Abstract

ABSTRACT: Development of an effective system for oocyte-cryopreservation is of clinical relevance in reproductive medicine. However, oocyte-preservation is not as effective as embryo preservation. In this study, we used a 37°C pre-equilibrium temperature as part of a modified vitrification method for human oocyte cryopreservation. The effect of the new method on spindle configuration, chromosomal arrangement, and mitochondrial distribution was investigated in in vitro -matured human oocytes. A total of 101 in vitro -matured oocytes were randomly assigned for vitrification at pre-equilibrium temperature of 37°C (37°C Group, n =50) or at room temperature (RT Group, 22-24°C, n =51). The time needed for vitrification in the 37°C group was significantly shorter than that in the RT group. Defective spindles were found in 45.5% and 69.0% oocytes in the 37°C group and RT group, respectively ( p < 0.05). Abnormal chromosomes were found in 47.7% and 71.4% oocytes, respectively ( p < 0.05). There were no significant differences with respect to oocyte survival rate and mitochondrial distribution pattern between the two groups. These results indicate that vitrification at a pre-equilibrium temperature of 37°C may reduce the incidence of defective spindle configuration and chromosomal abnormalities in in-vitro- matured human oocytes. Abbreviations : ICSI: intracytoplasmic sperm injection; FSH: follicle-stimulating hormone; MII: metaphase II; EG: ethylene glycol; PROH: 1, 2-propanediol