Salivary protein changes in response to acute stress in medical residents performing advanced clinical simulations: a pilot proteomics study. (19th May 2017)

• Record Type: Journal Article
• Authors: Marvin, Rachel K.; Saepoo, Muncharie B.; Ye, Simiao; White, Donald B.; Liu, Rong; Hensley, Kenneth; Rega, Paul; Kazan, Viviane; Giovannucci, David R.; Isailovic, Dragan
• Is Part Of: Biomarkers. Volume 22:Number 3/4(2017)
• Page Start: 372
• Page End: 382
• Publication Date: 2017-05-19
• Subjects: Saliva -- stress -- mass spectrometry -- gel electrophoresis -- amylase -- cystatins; Biochemical markers -- Periodicals; 610.28
• DOI: 10.1080/1354750X.2017.1279215
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Context: Quantitative changes of salivary proteins due to acute stress were detected. Objective: To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations. Materials and methods: Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry. Results: Salivary alpha-amylase and S–type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress. Discussion and conclusion: Alpha-amylase and cystatins are potential salivary markers of acute stress, but further validation should be performed using larger sample populations.