A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2, 4-dinitrophenolate. Issue 37 (24th March 2017)
- Record Type:
- Journal Article
- Title:
- A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2, 4-dinitrophenolate. Issue 37 (24th March 2017)
- Main Title:
- A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2, 4-dinitrophenolate
- Authors:
- Yevglevskis, Maksims
Lee, Guat L.
Nathubhai, Amit
Petrova, Yoana D.
James, Tony D.
Threadgill, Michael D.
Woodman, Timothy J.
Lloyd, Matthew D. - Abstract:
- Abstract : A versatile continuous colorimetric assay for AMACR is reported. Abstract : α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2, 4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and K i values, reversibility and characterizing irreversible inhibition.
- Is Part Of:
- Chemical communications. Volume 53:Issue 37(2017)
- Journal:
- Chemical communications
- Issue:
- Volume 53:Issue 37(2017)
- Issue Display:
- Volume 53, Issue 37 (2017)
- Year:
- 2017
- Volume:
- 53
- Issue:
- 37
- Issue Sort Value:
- 2017-0053-0037-0000
- Page Start:
- 5087
- Page End:
- 5090
- Publication Date:
- 2017-03-24
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c7cc00476a ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 39.xml