Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane. Issue 37 (25th April 2017)

• Record Type: Journal Article
• Authors: Clulow, James A.; Storck, Elisabeth M.; Lanyon-Hogg, Thomas; Kalesh, Karunakaran A.; Jones, Lyn H.; Tate, Edward W.
• Is Part Of: Chemical communications. Volume 53:Issue 37(2017)
• Page Start: 5182
• Page End: 5185
• Publication Date: 2017-04-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc08797c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Protein targets of sulforaphane identified, and their affinities quantified, through competition-based chemical proteomics in two live breast cancer cell lines. Abstract : Sulforaphane is a small molecule isothiocyanate which exhibits anticancer potential, yet its biological targets remain poorly understood. Here we employ a competition-based chemical proteomics strategy to profile sulforaphane's targets and identify over 500 targets along with their relative affinities. These targets provide a new set of mediators for sulforaphane's bioactivity, and aid understanding of its complex mode of action.