Bacteroidales markers for microbial source tracking in Southeast Asia. (1st July 2017)

• Record Type: Journal Article
• Authors: Nshimyimana, Jean Pierre; Cruz, Mercedes C.; Thompson, R. Janelle; Wuertz, Stefan
• Is Part Of: Water research. Volume 118(2017)
• Page Start: 239
• Page End: 248
• Publication Date: 2017-07-01
• Subjects: Fecal pollution -- Microbial source tracking -- Bacteroidales -- Quantitative PCR -- Singapore -- Southeast Asia; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2017.04.027
• Languages: English
• ISSNs: 0043-1354

Abstract

Abstract: The island city country of Singapore served as a model to validate the use of host-associated Bacteroidales 16S rRNA gene marker assays for identifying sources of fecal pollution in the urban tropical environment of Southeast Asia. A total of 295 samples were collected from sewage, humans, domesticated animals (cats, dogs, rabbits and chicken), and wild animals (birds, monkeys and wild boars). Samples were analyzed by real time PCR using five human-associated assays (HF183-SYBR Green, HF183, BacHum, BacH and B. thetaiotaomicron α-1-6, mannanase (B. theta), one canine-associated assay (BacCan), and a total Bacteroidales assay (BacUni). The best performing human-associated assay was B. theta with a diagnostic sensitivity of 69% and 100% in human stool and sewage, respectively, and a specificity of 98%. BacHum achieved the second highest sensitivity and specificity for human stool at 65% and 91%, respectively. The canine-associated Bacteroidales assay (BacCan) had a sensitivity and specificity above 80% and was validated for tracking fecal pollution from dogs. BacUni demonstrated a sensitivity and specificity of 100% for mammals, thus BacUni was confirmed for total Bacteroidales detection in the region. We showed for the first time that rabbit fecal samples cross-react with human-associated assays (HF183-SYBR Green, HF183, BacHum and BacH) and with BacCan. Our findings regarding the best performing human-associated assays differ from those reported in Bangladesh and India, which are geographically close to Southeast Asia, and where HF183 and BacHum were the preferred assays, respectively. Graphical abstract: Highlights: Validated Bacteroidales- associated assays applicable in Singapore and Southeast Asia. B. theta validated as the most specific of five human assays tested. Wild monkeys and boars did not cross-react with human-assays. Rabbit cross-reacted with all human assays except B. theta.