Triterpenoid saponins from the roots of Spergularia marginata. (July 2017)

• Record Type: Journal Article
• Authors: Pertuit, David; Larshini, Mustafa; Brahim, Malika Aitsidi; Markouk, Mohamed; Mitaine-Offer, Anne-Claire; Paululat, Thomas; Delemasure, Stéphanie; Dutartre, Patrick; Lacaille-Dubois, Marie-Aleth
• Is Part Of: Phytochemistry. Volume 139(2017)
• Page Start: 81
• Page End: 87
• Publication Date: 2017-07
• Subjects: Spergularia marginata -- Caryophyllaceae -- Triterpene saponins -- 2D NMR -- Cytotoxicity; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2017.03.007
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: Phytochemical investigations of the roots of Spergularia marginata had led to the isolation of four previously undescribed triterpenoid saponins, a known one and one spinasterol glycoside. Their structures were established by extensive NMR and mass spectroscopic techniques as 3- O -β-D-glucuronopyranosyl echinocystic acid 28- O -α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 3)-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 2)-α-L- arabinopyranosyl ester, 3- O -β-D-glucopyranosyl-(1 → 3)-β-D-glucuronopyranosyl echinocystic acid 28- O -α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 3)-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 2)- α-L-arabinopyranosyl ester, 3- O -β-D-glucopyranosyl-(1 → 4)-3- O -sulfate-β-D-glucuronopyranosyl echinocystic acid 28- O -α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 3)-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl ester, and 3- O -β-D-glucopyranosyl-(1 → 4)-β-D-glucuronopyranosyl 21- O -acetyl acacic acid. Their cytotoxicity was evaluated against two human cancer cell lines SW480 and MCF-7. The most active compound showed a cytotoxicity with IC50 14.2 ± 0.8 μ M (SW480), and 18.7 ± 0.8 μ M (MCF-7), respectively. Graphical abstract: Phytochemical investigations of the roots of Spergularia marginata had led to the isolation of four previously undescribed triterpenoid saponins, a known one and one spinasterol glycoside. There structures were established by spectroscopic methods. Compounds were tested for their cytotoxic effects. Highlights: Four triterpenoid saponins were isolated from Spergularia marginata. Structures were elucidated by spectroscopic methods. Cytotoxicity was evaluated against SW480 and MCF-7 cancer cell lines.