Anti-inflammatory activity of Crateva adansonii DC on keratinocytes infected by Staphylococcus aureus: From traditional practice to scientific approach using HPTLC-densitometry. (23rd May 2017)

• Record Type: Journal Article
• Authors: Ahama-Esseh, Kplolali; Bodet, Charles; Quashie-Mensah-Attoh, Akossiwa; Garcia, Magali; Théry-Koné, Isabelle; Dorat, Joelle; De Souza, Comlan; Enguehard-Gueiffier, Cécile; Boudesocque-Delaye, Leslie
• Is Part Of: Journal of ethnopharmacology. Volume 204(2017)
• Page Start: 26
• Page End: 35
• Publication Date: 2017-05-23
• Subjects: AUC area under the curve -- BPE Bovine pituitary extract -- DPPH 2, 2-diphenyl-1-picrylhydrazyl free radical -- CH2Cl2 methylene chloride -- DNI direct normal irradiation -- EDTA ethylene diamine tetraacetic acid -- EGF epidermal growth factor -- EtOAc ethyl acetate -- HPTLC High Performance Thin Layer Chromatography -- ICH International Conference of Harmonization -- IL6 Interleukine 6 -- IL8 Interleukine 8 -- K-SFM Keratinocyte-Serum Free Medium -- LOD limit of detection -- LOQ limit of quantification -- MAPK mitogen activated protein kinase -- MeOH methanol -- MOI multiplicity of infection -- MTT 3-(4, 5-methylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- n-BuOH n-butanol -- NF-κB nuclear factor-kappa B -- PBS phosphate buffer saline -- PVGIS PhotoVoltaic Geographical Information System -- r2 correlation coefficient -- RDSC Radical DPPH Scavenging Capacity -- rpm round per minutes -- RSD Relative standard deviation -- RT-PCR real time polymerase chain reaction -- SEM standard error of the mean -- TE trolox equivalent -- TLC Thin Layer Chromatography -- TNFα Tumor Necrosis Factor α -- v/v volume/volume -- XTT 3′-{1-[(phenylamino)carbonyl]-3, 4-tetrazolium}-bis(4-methoxy-6-nitro)benzene sulfonic acid hydrate -- w/w weigh/weigh; Traditional medicine Africa -- Inflammation -- Antioxidant -- Chromatography -- Skin; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2017.04.001
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacoligical relevance: Leaves of Crateva adansonii DC (Capparidaceae), a small bush found in Togo, are widely used in traditional medicine to cure infectious abscesses. Traditional healers of Lomé harvest only budding leaves early in the morning, in specific area in order to prepare their drugs. Aim of the study: The main goal was to validate the ancestral picking practices, and to assess the activity of C. adansonii medicine towards infectious abscesses. Materials and methods: A phytochemical screening of various C. adansonii leaf samples was performed using an original HPTLC-densitometry protocol and major flavonoids were identified and quantified. C. adansonii samples were collected in different neighborhoods of Lomé, at different harvesting-times and at different ages. Radical scavenging capacity, using DPPH assay, was used to quickly screen all extracts. Extracts were tested for anti- Staphylococcus aureus activity and anti-inflammatory effect on human primary keratinocytes infected by S. aureus . IL6, IL8 and TNFα expression and production were assessed by RT-PCR and ELISA assays. Results: Using antioxidant activity as selection criteria, optimal extracts were obtained with budding leaves, collected at 5:00 am in Djidjolé neighborhood. This extract showed the strongest anti-inflammatory effect on S. aureus -infected keratinocytes by reducing IL6, IL8 and TNFα expression and production. None of the extracts inhibited the growth of S. aureus . Conclusions: Those results validate the traditional practices and the potential of C. adansonii as anti-inflammatory drug. Our findings suggest that traditional healers should add to C. adansonii leaves an antibacterial plant of Togo Pharmacopeia, in order to improve abscess healing. Graphical abstract: