Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake. Issue 6 (May 2017)

• Record Type: Journal Article
• Authors: Soldánová, Miroslava; Georgieva, Simona; Roháčová, Jana; Knudsen, Rune; Kuhn, Jesper A.; Henriksen, Eirik H.; Siwertsson, Anna; Shaw, Jenny C.; Kuris, Armand M.; Amundsen, Per-Arne; Scholz, Tomáš; Lafferty, Kevin D.; Kostadinova, Aneta
• Is Part Of: International journal for parasitology. Volume 47:Issue 6(2017)
• Page Start: 327
• Page End: 345
• Publication Date: 2017-05
• Subjects: Trematode diversity -- Intermediate hosts -- Phylogeny -- Mitochondrial DNA -- Nuclear DNA -- Lake Takvatn -- Norway -- Sub-Arctic; Parasitology -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; Parasitology; Periodicals; Electronic journals; 571.999
• DOI: 10.1016/j.ijpara.2016.12.008
• Languages: English
• ISSNs: 0020-7519

Abstract

Graphical abstract: Highlights: First known estimates of trematode diversity in a sub-Arctic lake. Molecular characterisation of 120 digenean isolates from aquatic hosts in Takvatn. Phylogenies based on nuclear and/or mitochondrial DNA. High digenean diversity: 24 species/species-level genetic lineages. Species assemblages unique to sub-Arctic and Arctic ecosystems. Abstract: To identify trematode diversity and life-cycles in the sub-Arctic Lake Takvatn, Norway, we characterised 120 trematode isolates from mollusc first intermediate hosts, metacercariae from second intermediate host fishes and invertebrates, and adults from fish and invertebrate definitive hosts, using molecular techniques. Phylogenies based on nuclear and/or mtDNA revealed high species richness (24 species or species-level genetic lineages) and uncovered trematode diversity (16 putative new species) from five families typical in lake ecosystems (Allocreadiidae, Diplostomidae, Plagiorchiidae, Schistosomatidae and Strigeidae). Sampling potential invertebrate hosts allowed matching of sequence data for different stages, thus achieving molecular elucidation of trematode life-cycles and exploration of host-parasite interactions. Phylogenetic analyses also helped identify three major mollusc intermediate hosts ( Radix balthica, Pisidium casertanum and Sphaerium sp.) in the lake. Our findings increase the known trematode diversity at the sub-Arctic Lake Takvatn, showing that digenean diversity is high in this otherwise depauperate sub-Arctic freshwater ecosystem and indicating that sub-Arctic and Arctic ecosystems may be characterised by unique trematode assemblages.