Ectonucleotidase and adenosine deaminase as inflammatory marker in dairy cows naturally infected by Dictyocaulus viviparus. (April 2017)

• Record Type: Journal Article
• Authors: da Silva, Aleksandro S.; Baldissera, Matheus D.; Bottari, Nathieli B.; Doleski, Pedro H.; Carmo, Guilherme M.; Schwertz, Claiton I.; Lucca, Neuber J.; Henker, Luan C.; Piva, Manoela M.; Giacomin, Patrícia; Stefani, Lenita M.; Leal, Daniela B.R.; Machado, Gustavo; Mendes, Ricardo E.
• Is Part Of: Comparative immunology, microbiology and infectious diseases. Volume 51(2017)
• Page Start: 9
• Page End: 13
• Publication Date: 2017-04
• Subjects: Dictyocaulosis -- Purinergic system -- Treatment -- Immune response; Communicable diseases in animals -- Periodicals; Veterinary immunology -- Periodicals; Veterinary microbiology -- Periodicals; Immunology -- Periodicals; Microbiology -- Periodicals; Communicable diseases -- Periodicals; Communicable Diseases -- immunology -- Periodicals; Communicable Diseases -- veterinary -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Veterinary Medicine -- Periodicals; Immunologie -- Périodiques; Microbiologie -- Périodiques; Maladies infectieuses -- Périodiques; Communicable diseases; Immunology; Microbiology; Electronic journals; Periodicals; 636.08969
• DOI: 10.1016/j.cimid.2017.01.001
• Languages: English
• ISSNs: 0147-9571

Abstract

Highlights: Dictyocaulus viviparus is the causative agent of dictyocaulosis in cattle, also known as parasitic bronchitis. The dictyocaulosis alters NTPDase, 5′-nucleotidase, and ADA activities in naturally infected dairy cows. The purinergic signaling pathway might be related to the inflammation process caused by disease. Post-treatment reduced the activity of NTPDase to ATP and ADP substrate. Post-treatment increased the activities of enzymes 5′-nucleotidase and adenosine deaminase. Abstract: The aim of this study was to evaluate the influence of dictyocaulosis (mild or severe) on enzymes of NTPDase, 5′-nucleotidase, and adenosine deaminase (ADA) of dairy cows naturally infected by Dictyocaulus viviparus. Blood and faeces were collected from 22 dairy cows of the same farm to evaluate NTPDase (ATP and ADP substrate), 5′-nucleotidase, and ADA activities on days 0 (pre-treatment) and 10 (post-treatment). Seric activities of NTPDase (ATP substrate), 5′-nucleotidase, and ADA were lower ( P < 0.05) in D. viviparus infected animals compared to uninfected cows. The number of D. viviparus larvae per gram of faeces varied among the animals, and they showed different degrees of severity according to respiratory clinical signs of the disease (cough and nasal discharge). Later, these cows were divided into two groups: those with mild (n = 10) and severe (n = 12) disease. Cows with severe disease showed higher NTPDase activity (ATP substrate) than those with mild disease (P ≤ 0.05). The opposite occurred with NTPDase (ADP substrate), 5′-nucleotidase, and ADA in cows with severe disease, that is, the enzymatic activity of these seric enzymes significantly decreased (P ≤ 0.05) compared to animals with mild disease. Infected animals showed reduced NTPDase activity (ATP and ADP substrate) after treatment. No enzymatic changes were observed for 5′-nucleotidase, and ADA pre- and post-treatment (P > 0.05). Based on these results, we conclude that dictyocaulosis alters NTPDase, 5′-nucleotidase, and ADA activities of cow naturally infected by the parasite, in consequence the enzymes act as inflammatory markers.