Acetylcholine-induced ex vivo ATP release from the human nasal mucosa. Issue 4 (August 2017)

• Record Type: Journal Article
• Authors: Koizumi, Hiroki; Ikezaki, Shoji; Ohbuchi, Toyoaki; Do, Ba Hung; Hohchi, Nobusuke; Kawaguchi, Rintaro; Kitamura, Takuro; Suzuki, Hideaki
• Is Part Of: Auris nasus larynx. Volume 44:Issue 4(2017)
• Page Start: 422
• Page End: 427
• Publication Date: 2017-08
• Subjects: Ach acetylcholine -- M3AchR M3 acetylcholine receptor -- PLC phospholipase C -- PIP2 phosphatidylinositol bisphosphate -- DAG diacylglycerol -- IP3 inositol triphosphate -- IP3R inositol triphosphate receptor -- P2X7 purinergic P2X7 receptor; Human nasal mucosa -- Inferior turbinate -- ATP -- Acetylcholine -- Pannexin-1 -- P2X7 receptor; Otolaryngology -- Periodicals; Electronic journals; 616
• DOI: 10.1016/j.anl.2016.09.003
• Languages: English
• ISSNs: 0385-8146

Abstract

Abstract: Objective: The present study aimed at investigating ATP release in response to acetylcholine (Ach) and pharmacologically elucidating the intracellular signal transduction pathway of this reaction in an ex vivo experiment. Methods: The inferior turbinate mucosa was collected from 21 patients with chronic hypertrophic rhinitis who underwent endoscopic turbinectomy. The mucosa was shaped into a filmy round piece, and incubated with chemical(s) in Hank's balanced salt solution for 10 min. After incubation, the ATP concentration was measured by a luciferin-luciferase assay. Results: The baseline release of ATP without stimulus was 57.2 ± 10.3 fM. The ATP release was significantly increased by stimulation with 100 μM Ach. The Ach-induced ATP release was completely inhibited by removing extracellular Ca 2+ . Significant inhibition of the Ach-induced ATP release was also observed by the addition of 1 μM atropine, 40 μM 2-APB, 10 μM CBX, and 100 μM PPADS, whereas 30 nM bafilomycin A1 did not affect the ATP release. Conclusion: These results indicate that the Ach-induced ATP release from the human nasal mucosa is dependent on the pannexin-1 channel and purinergic P2X7 receptor, suggesting that these two molecules constitute a local autocrine/paracrine signaling system in the human nasal epithelium.