Transplanting mouse induced pluripotent stem cells into mouse otocysts in vivo. (24th April 2017)

• Record Type: Journal Article
• Authors: Takeda, Hiroki; Minoda, Ryosei; Miwa, Toru; Yamada, Takao; Ise, Momoko
• Is Part Of: Neuroscience letters. Volume 647(2017)
• Page Start: 153
• Page End: 158
• Publication Date: 2017-04-24
• Subjects: Cell transplantation -- iPS -- Otocyst -- Connexin 30 -- Tumorigenesis; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2017.03.014
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Transplanted mouse iPS cells survived and differentiated in the developing mouse inner ears. Transplanted mouse iPS cells migrated in the developing inner ear epitheliums in Cx30-deficient mice. Tumorigenesis was observed in the Cx30-knockout mice but not in the WT mice. Abstract: The otocyst is an attractive target for studying treatment strategies for genetic hearing loss and for understanding inner ear development. We have previously reported that trans-uterine supplemental gene therapy in vivo into the otocysts of mice, which had a loss of function mutation in a causative gene of deafness, was able to prevent putative hearing loss. We herein set out to clarify the feasibility of allogenic cell transplantation into the mouse otocysts in vivo. We transplanted naive mouse-derived induced pluripotent stem cells (miPSCs) into the otocysts of wild type mice or connexin (Cx) 30 deficient mice, at embryonic day 11.5 (E11.5). The transplanted m-iPSCs survived in the lumens of the inner ears at E13.5 and E15.5 in wild type mice. In the Cx30 deficient mouse, the transplanted cells survived similarly, with some of the transplanted cells migrating into the lining cells of the lumens of the inner ears at E13.5 and showing tumorigenic cell proliferation at E15.5. In addition, engrafted cells appear to be able to differentiate after the cell transplantation. Our results suggest that otocyst transplanted cells survived and differentiated. A Cx30 deficiency may facilitate cell migration. These findings may offer some hope for cell transplantation therapy for profound genetic hearing loss caused by a Cxs deficiency.