FVIIa prevents the progressive hemorrhaging of a brain contusion by protecting microvessels via formation of the TF–FVIIa–FXa complex. (21st April 2017)

• Record Type: Journal Article
• Authors: Yuan, Qiang; Zhang, Dalong; Wu, Sirong; Yu, Jian; Yu, Lei; Sun, Yirui; Du, Zhuoying; Li, Zhiqi; Zhou, Liangfu; Wu, Xing; Hu, Jin
• Is Part Of: Neuroscience. Volume 348(2017)
• Page Start: 114
• Page End: 125
• Publication Date: 2017-04-21
• Subjects: APC anticoagulant protein C/activated protein C -- CCI controlled cortical impact -- CT computed tomography -- ELISA enzyme-linked immunosorbent assay -- EPCR endothelial cell protein C receptor -- FVII factor VII -- GPCR G protein-coupled receptor -- HUVECs human umbilical vein endothelial cells -- IgG Immunoglobulin G -- MAPK mitogen-activated protein kinase -- mFVII mouse FVII -- MRI magnetic resonance imaging -- NE neutrophil elastase -- NF-κB nuclear factor-κB -- PAR2 protease-activated receptor 2 -- PKC protein kinase C -- PR3 neutrophil proteinase-3 -- TBI traumatic brain injury -- TEs thromboembolic events -- TF tissue factor; FVIIa -- TF–FVIIa–FXa complex -- endothelial cell -- progressive hemorrhage -- brain contusion; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2017.02.020
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: The decrease of FVII activity is closely related to the increase of brain contusion hemorrhage size. FVIIa prevents the progressive hemorrhaging of brain contusions by protecting microvessel endothelial cells. Ternary TF–FVIIa–FXa protects endothelial cells through activating p44/42 MAPK and inhibiting p65 NF-κB signaling pathway. Abstract: Factor VII (FVII) plays a key role in the initiation of the coagulation cascade and, in clinical situations, recombinant human activated FVII (rFVIIa) effectively prevents progressive hemorrhaging after a brain contusion. However, it remains unclear whether decreases in FVII activity directly lead to progressive hemorrhaging and, moreover, the precise mechanisms underlying this process are not yet known. The present study demonstrated that decreased FVII activity directly led to progressive hemorrhaging of the cerebral contusions. Administration of FVII prevented the progression of hemorrhaging from cerebral contusions by protecting microvessel endothelial cells in the penumbra of the contusion. The present study also showed that the ternary TF–FVIIa–FXa complex cleaved endogenous protease-activated receptor 2 (PAR2) on endothelial cells, activated the p44/42 mitogen-activated protein kinase (MAPK) signaling cascade, and inhibited p65 nuclear factor-κB (NF-κB) signaling. Furthermore, exposure to ternary TF–FVIIa–FXa protected endothelial cells from thrombin- or inflammatory cytokine-induced apoptosis. Although activation of the p44/42 MAPK signaling pathway is endothelial cell protein C receptor (EPCR)-dependent, inhibition of the p65 NF-κB signaling pathway is EPCR-independent; thus, the regulation mechanism underlying the effects of TF–FVIIa–FXa in vascular endothelial cells appears to be multiple signaling pathways. In summary, the present findings demonstrated that FVIIa prevented the progressive hemorrhaging of brain contusions by protecting microvessel endothelial cells via the formation of the ternary TF–FVIIa–FXa complex. These findings are novel and of great clinical significance because FVIIa is used to prevent the progressive hemorrhaging of brain contusions in humans.