Application of fluorescence polarization immunoassay for determination of carbamazepine in wastewater. (15th May 2017) - Oberleitner, Lidia
Abstract: Carbamazepine is an antiepileptic drug that can be used as a marker for the cleaning efficiency of wastewater treatment plants. Here, we present the optimization of a fast and easy on-site measurement system based on fluorescence polarization immunoassay and the successful application to wastewater. A new monoclonal highly specific anti-carbamazepine antibody was applied. The automated assay procedure takes 16 min and does not require sample preparation besides filtration. The recovery rates for carbamazepine in wastewater samples were between 60.8 and 104% with good intra- and inter-assay coefficients of variations (less than 15 and 10%, respectively). This automated assay enables for the on-site measurement of carbamazepine in wastewater treatment plants. Graphical abstract: Highlights: Carbamazepine is determined with a homogeneous fluorescence polarization immunoassay. High precision was obtained. The procedure of the mix-and-read assay was optimized to cope with the complex matrix. No tedious sample clean-up is required for wastewater.