Fabrication and evaluation of thermosensitive chitosan/collagen/α, β-glycerophosphate hydrogels for tissue regeneration. (1st July 2017)

• Record Type: Journal Article
• Authors: Dang, Qifeng; Liu, Kai; Zhang, Zhenzhen; Liu, Chengsheng; Liu, Xi; Xin, Ying; Cheng, Xiaoyu; Xu, Tao; Cha, Dongsu; Fan, Bing
• Is Part Of: Carbohydrate polymers. Volume 167(2017)
• Page Start: 145
• Page End: 157
• Publication Date: 2017-07-01
• Subjects: Chitosan (PubChem CID: 71853) -- α-glycerophosphate (PubChem CID: 14754) -- β-glycerophosphate (PubChem CID: 6101544) -- Acetic Acid (PubChem CID: 176) -- MTT (PubChem CID: 64965) -- Calcein-AM (PubChem CID: 4126474) -- Sodium chloride (PubChem CID: 5234) -- Potassium bromide (PubChem CID: 253877); Chitosan -- Acid-soluble collagen -- Thermosensitive hydrogel -- Characterization -- Biocompatibility -- 3D cell culture; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.03.053
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: CS-ASC-HGs were successfully prepared and had excellent thermosensitivity. CS-ASC-HGs possessed 3D porous architectures containing fibrous ASC. CS-ASC-HGs had better pH stability and biocompatibility than pure CS-HG. CS-ASC-HGs could serve as 3D cell culture matrices for L929. Abstract: Thermosensitive hydrogels whose physiological properties are similar to extracellular matrix have been extensively used for tissue regeneration. Polysaccharides and proteins, as biocompatible substrates similar to bio-macromolecules that could be recognized by human body, are two preferred polymers for fabrication of such hydrogels. A series of novel thermosensitive hydrogels (CS-ASC-HGs) containing chitosan (CS) and acid-soluble collagen (ASC) were thus prepared, in the presence of α, β-glycerophosphate, to mimic extracellular microenvironment for tissue regeneration. Rheological measurements demonstrated excellent thermosensitivity. FT-IR and SEM indicated CS-ASC-HGs possessed 3D porous architectures with fibrous ASC, and the molecular structure of ASC was well-maintained in hydrogels. Hemolysis, acute toxicity, and cytotoxicity tests suggested CS-ASC-HGs were of good biocompatibility. CS-ASC-HGs were able to support the survival and proliferation of L929 cells encapsulated in them. Moreover, CS-ASC-HGs had better pH stability and biocompatibility than pure CS hydrogel. These results suggested that CS-ASC-HGs could serve as promising scaffolds for tissue regeneration.