Quercetin protects against heat stroke-induced myocardial injury in male rats: Antioxidative and antiinflammatory mechanisms. (1st March 2017)

• Record Type: Journal Article
• Authors: Lin, Xiaojing; Lin, Cheng-Hsien; Zhao, Tingbao; Zuo, Dan; Ye, Zhujun; Liu, Lin; Lin, Mao-Tsun
• Is Part Of: Chemico-biological interactions. Volume 265(2017)
• Page Start: 47
• Page End: 54
• Publication Date: 2017-03-01
• Subjects: Heat stroke -- Myocardial injury -- Quercetin -- Oxidative stress -- Inflammation; MAP mean arterial pressure -- HR heart rate -- NC normothermic controls -- V vehicle -- Q quercetin -- HS heat stroke -- PBS phosphate buffered saline -- CK creatine kinase -- PV pressure-volume -- CV stroke volume -- EDP left ventricle (LV) end-diastolic pressure -- ESP LV end-systolic pressure -- Pmin minimum LV pressure -- Pmax maximum LV pressure -- EDV LV end-diastolic volume -- ESV LV end-systolic volume -- Vmax maximum dv/dt -- Vmin minimum dv/dt -- SW stroke work -- Co cardiac output -- Ea arterial elastance -- Tau (γ) Glantz time constant of ventricular relaxation -- EF ejection fraction -- TBARS Thiobarbituric acid reactive substances -- SOD superoxide dis-mutase -- GSH reduced glutathione -- TNF-α tumor necrosis factor-α -- IL-6 interleukin-6 -- IL-10 interleukin-10; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2017.01.006
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Heat stroke is characterized by hyperthermia, systemic inflammation, and multiple organ failure including arterial hypotension. This definition can be fulfilled by a rat model of heat stroke used in the present study. Anesthetized animals were exposed to heat exposure (43 °C for 70 min) and then returned to room temperature (26 °C) for recovery. One hour before heat exposure, an intraperitoneal dose of quercetin (30 mg/kg) or vehicle (normal saline 1 ml/kg) was administered to the experimental groups of rats. Additional injection was administered immediately after the onset of heat stroke. Immediately after the onset of heat stroke. Vehicle-treated rats displayed (i) hyperthermia; (ii) suppressed left ventricular function; (iii) decreased contents of cardiac total antioxiant capacity (e.g., superoxide dismutase, glutathione peroxidase, catalase); (iv) increased contents of cardiac oxidative capacity malondialdehyde and thiobarbituric acid reactive substances; (v) increased cardiac levels of pro-inflammatory cytokines tumor necrosis factor-α and interleukin-6; and (vi) decreased cardiac levels of an anti-inflammatory cytokine interleukin 10. Histopathologic and survival observation provided supportive evidence for biochemical analyses. These heat stroke reactions all can be significantly attenuated by quercetin therapy. Our data suggest that quercetin therapy might improve outcomes of heat stroke in rats by attenuating excessive hyperthermia as well as myocardial injury. The protective effects of quercetin could be attributed to anti-lipid peroxidative, anti-oxidant, and anti-inflammatory properties. Highlights: Heat stroke male rats displayed hyperthermia, hypotension, and myocardial inflammation and oxidative stress. Hypothermia is related to myocardial inflammation and oxidative injury in heat stroke. Quercetin protects against heat stroke-induced hyperthermia, hypotension, and myocardial injury. Quercetin may exert its protection via anti-oxidant and anti-inflammatory action.