Mucopolysaccharide from cuttlefish: Purification, chemical characterization and bioactive potential. (1st July 2017)

• Record Type: Journal Article
• Authors: Seedevi, Palaniappan; Moovendhan, Meivelu; Vairamani, Shanmugam; Shanmugam, Annaian
• Is Part Of: Carbohydrate polymers. Volume 167(2017)
• Page Start: 129
• Page End: 135
• Publication Date: 2017-07-01
• Subjects: Sulfated glycosaminoglycans -- Anticoagulant activity -- Anticancer; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.03.028
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: The Uronic acid was contributed more it as 50.11%. The Sulfate was found higher it as 27.69%. The molecular weight of GAG was calculated as 17 kDa. The sulfated GAG has showed promising anticoagulant and anticancer activities. Abstract: The sulfated mucopolysaccharide (GAG) was isolated from S. pharonis and the carbohydrate and protein content was found to be 62.4% and 3.9%. The disaccharide profile of sulfated GAG composed glucuronic acid, N-acetyl glucosamine and sulfate content by contributing 50.11%, 38.00% and 27.69% respectively. The carbon, hydrogen and nitrogen content of the sulfated GAG showed 14.80%, 1.68% and 2.99% respectively. The molecular weight of sulfated GAG was calculated as 27 kDa and the structural characterization was done by Fourier Transform Infrared (FT-IR) and NMR Spectroscopy. The Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) of sulfated GAG were determined as 91 IU and 39.55 IU at 25 μg/ml respectively. Further the sulfated GAG reported the cytotoxic effect (CC50 ) of 1100 μg/ml concentration on Vero cell line. The sulfated GAG reported the anticancer activity against HeLa cell line with an inhibition rate of 18.65%–66.13% at 50–250 μg/ml concentration. The sulfated GAG can be considered as a potent anticoagulant and anticancer drug in future.