Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells. (1st July 2017)

• Record Type: Journal Article
• Authors: Sun, Pengda; Sun, Dong; Wang, Xudong
• Is Part Of: Carbohydrate polymers. Volume 167(2017)
• Page Start: 90
• Page End: 96
• Publication Date: 2017-07-01
• Subjects: Scutellaria barbata polysaccharide -- Proliferation -- Apoptosis -- Epithelial-mesenchymal transition -- Colon cancer; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.03.022
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A homogeneous polysaccharide (SPS2p) was isolated from Scutellaria barbata. . SPS2p could improve the proliferation inhibition rate of HT29 cells. SPS2p could elevate the apoptosis rate and apoptosis index. SPS2p could elevate the levels of Bax and Bak, but lower level of Bcl-2. SPS2p could reduce the ratio of p-AKT/AKT. Abstract: A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins. The molecular weight of SPS2p showed only one molecular weight distribution (2.6 × 10 4 Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59. The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells. These results indicate that SPS2p can inhibit the proliferation and EMT, and promote the apoptosis in HT29 cells, which may be related to the inhibition of SPS2p on the PI3K/AKT pathway.